TRI Reagent总RNA提取试剂-sigma

2018年9月30日10:36:07 评论

产品描述TRI Reagent总RNA提取试剂-sigma


TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski. The RNA isolation method based on this reagent is widely used and proven for RNA applications. It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA, and proteins from samples of human, animal, plant, yeast, bacterial, and viral origin.

The reagent has been used for isolating RNA.[1][2][3] The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assay, cloning, and PCR.

Features and Benefits

• Easily scalable RNA isolation
• Works with many sources: human, plant, yeast, bacterial, or viral
• Better yields than traditional guanidine thiocyanate/cesium chloride methods


25, 100, 200 mL in glass bottle

General description

TRI Reagent is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA, and protein. Successful isolations from human, animal, plant, yeast, bacterial, and viral samples can be obtained. A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA, and protein. This procedure is an improvement of the single-step method reported by Chomczynski and Sacchi for total RNA isolation. TRI Reagent performs well with large or small amounts of tissue or cells and many samples can be simultaneously extracted.

This product, a mixture of guanidine thiocyanate and phenol in a monophase solution, effectively dissolves DNA, RNA, and protein on homogenization or lysis of tissue sample. After adding chloroform or 1-bromo-3-chloropropane and centrifuging, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA, and an organic phase containing proteins. Each component can then be isolated after separating the phases. One ml of TRI Reagent is sufficient to isolate RNA, DNA, and protein from 50-100 mg of tissue, 5-10 ´ 106 cells, or 10 cm2 of culture dish surface for cells grown in monolayer.

This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh tissue or cells. The procedure is very effective for isolating RNA molecules of all types from 0.1-15 kb in length. The resulting RNA is intact with little or no contaminating DNA and protein.


TRI Reagent 为以下机构的注册商标: Molecular Research Center, Inc.






  • 我的微信
  • 微信扫一扫
  • weinxin
  • 我的微信公众号
  • 微信扫一扫
  • weinxin
  • 本文由 发表于 2018年9月30日10:36:07
  • 转载请务必保留本文链接:
大型野生动物遥测项圈Vertex Plus 土壤养分

大型野生动物遥测项圈Vertex Plus

Vertex Plus项圈是全球野生动物科学领域常用的GPS项圈。它基于全新的电子和硬件设置,使用成熟的包装和绑缚皮带设计。它在电池利用效率、数据准确性、数据的传输以及野外可靠性等方面都有成熟的表现。...


匿名网友 填写信息

:?: :razz: :sad: :evil: :!: :smile: :oops: :grin: :eek: :shock: :???: :cool: :lol: :mad: :twisted: :roll: :wink: :idea: :arrow: :neutral: :cry: :mrgreen: